1999•Journal of Veterinary Medicine Series BRequires access

A Screening ELISA for Brucellosis in Reindeer

Kjetil Åsbakk, D. Gall, Snorre Stuen

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the screening of brucellosis in reindeer was developed. The assay, which utilizes s-LPS from Brucella abortus as antigen and biotin-labelled rabbit antibody to reindeer immunoglobulin as detecting antibody, has a high specificity and sensitivity, as indicated in a validation with sera from reindeer cultured positive for Brucella suis biovar 4 and sera from reindeer free of brucellosis.

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Key concepts: Brucellosis, Brucella abortus, Brucellaceae, Biovar, Brucella, Brucella suis, Antibody, Antigen