2007•Current Protocols in ImmunologyRequires access
Generation of Epstein‐Barr Virus (EBV)–Immortalized B Cell Lines
Giovanna Tosato, Jeffrey I. Cohen
Open publisher page 136 citations
Abstract
Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.