1999•Encyclopedia of Bioprocess TechnologyRequires access
Enzymes, Directed Evolution
Frances H. Arnold, Patrick L. Wintrode
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Abstract
Introduction
Why Directed Evolution?
The Limitations of Nature's Biocatalysts Present Insurmountable Challenges to Rational Design
Extending Natural Diversity by Laboratory Evolution
Choosing an Evolutionary Strategy
Good Problems for Directed Enzyme Evolution
Methods of Directed Evolution
Methods for Generating Genetic Diversity
Searching for Improved Enzymes
Extracting Useful Information from the Results of Evolution
Examples of Directed Evolution Applications
Evolution of Lipases with High Activity in Detergent Solutions
Evolution of an Esterase for Deprotection of p-Nitrobenzyl Esters and Enhanced Thermostability
Evolution of Herpes Simplex Virus Thymidine Kinase for Gene Therapy
Evolution of Biphenyl Dioxygenases for Bioremediation of PCBs
Acknowledgments
Bibliography
Keywords:
biocatalysis;
catalyst improvement;
DNA shuffling;
high-throughput screening;
protein engineering;
random mutagenesis;
recombination;
selection