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2001•Current Protocols in Molecular BiologyRequires access

HPLC of Peptides and Proteins: Preparation and System Set‐Up

Reinhard I. Boysen, Milton T. W. Hearn

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Abstract

The eight basic modes of HPLC currently in use for peptide and protein analysis and purification are presented in this overview. They include: size-exclusion chromatography (HP-SEC), ion-exchange chromatography (HP-IEX), normal phase chromatography (HP-NPC), hydrophobic interaction chromatography (HP-HIC), reversed-phase chromatography (RP-HPLC), hydrophilic interaction chromatography (HP-HILIC), immobilized metal ion affinity chromatography (HP-IMAC), and biospecific/biomimetic affinity chromatography (HP-BAC). In addition, some subsets of these chromatographic modes, e.g., mixed mode chromatography (HP-MMC), charge transfer chromatography (HP-CTC), or ligand-exchange chromatography (HP-LEC) are described. The unit includes overviews of the system requirements and planning strategies for set-up of the systems.

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What this paper is about

The eight basic modes of HPLC currently in use for peptide and protein analysis and purification are presented in this overview. They include: size-exclusion chromatography (HP-SEC), ion-exchange chromatography (HP-IEX), normal phase chromatography (HP-NPC), hydrophobic interaction chromatography (HP-HIC), reversed-phase chromatography (RP-HPLC), hydrophilic interaction chromatography (HP-HILIC), immobilized metal ion affinity chromatography (HP-IMAC), and biospecific/biomimetic affinity chromatography (HP-BAC). In addition, some subsets of these chromatographic modes, e.g., mixed mode chromatography (HP-MMC), charge transfer chromatography (HP-CTC), or ligand-exchange chromatography (HP-LEC) are described. The unit includes overviews of the system requirements and planning strategies for set-up of the systems.

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Available abstract

The eight basic modes of HPLC currently in use for peptide and protein analysis and purification are presented in this overview. They include: size-exclusion chromatography (HP-SEC), ion-exchange chromatography (HP-IEX), normal phase chromatography (HP-NPC), hydrophobic interaction chromatography (HP-HIC), reversed-phase chromatography (RP-HPLC), hydrophilic interaction chromatography (HP-HILIC), immobilized metal ion affinity chromatography (HP-IMAC), and biospecific/biomimetic affinity chromatography (HP-BAC). In addition, some subsets of these chromatographic modes, e.g., mixed mode chromatography (HP-MMC), charge transfer chromatography (HP-CTC), or ligand-exchange chromatography (HP-LEC) are described. The unit includes overviews of the system requirements and planning strategies for set-up of the systems.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Computational biology, Biochemistry, Biology

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