2007•PubMedRequires access
[Optimization of submerged culture condition for production of mycelial biomass by Armillaria mellea].
Xianhao Cheng, Linde Liu, Hong-Xin Dong, QU Hui-ge, Cai De-hua
Open publisher page 2 citations
Abstract
OBJECTIVE: To optimize the submerged culture conditions for the production of mycelial biomass by Amillaria mellea. METHODS: Using the statistically based experimental design in a shake flask culture, optimum concentration of each medium component was determined using the statistical method. RESULTS: Dextrin was the suitable carbon source, bean cake extract was the suitable nitrogen source. Both corn steep liquor and thiamin favored the mycelial growth. Ethanol also favored the mycelial growth. (NH4 )2SO4 and NaNO3 inhibited mycelial growth whereas KH2PO4 favored the mycelial growth. CONCLUSION: The optimal combination of the media concentrations for mycelial growth was as follows: bean cake extract 25%, corn steep liquor 2%, dextrin 2.5%, thiamin 0.06%, ethanol 1.0%, KH2PO4 0.3%, pH 6.0. Under the optimal culture condition, the production of mycelial biomass achieved 1.9g/100ml.