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Storage of strawberry plants in vitro, their stability and economical aspects.

Ivan Mikhailovich Kulikov, V. G. Vysotskiy

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Abstract

Economical efficiency of different in vitro techniques depends on botanical species and the scale of production. We have shown that the profitability of micropropagation for commercial laboratories can achieve 160%. In system of healthy plant production sufficient costs are charged on regular virology testing. It is possible to avoid partly of these costs by means of keeping virus-free plants in vitro. We have investigated the influence of strawberry long-term storage on propagation and rooting capacity, appearance of non-true type regenerants, pollen fertility, yielding capacity and vegetative productivity. It was shown the relatively high stability of the main pomological characters of strawberry plants after 15 years of storage. For economical evaluations we have compared methods of deposition in vitro and the maintenance of usual field collections. For this purpose the expenses of both treatments were counted. Calculations were made for 50 varieties (20 copies per each variety, total quantity 1000 copies). According to our calculations, the storage of strawberry in vitro in refrigerating chambers is 3.2 times more economical than maintenance of field collection. Besides, there is significant economy of the soil areas. Also it is necessary to note, that stored in vitro material does not reduce the phytosanitary status. According to our researches, plants after in vitro storage produced more runner plants in comparison with traditionally propagated ones. Thus the potential... [to full text]

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What this paper is about

Economical efficiency of different in vitro techniques depends on botanical species and the scale of production. We have shown that the profitability of micropropagation for commercial laboratories can achieve 160%. In system of healthy plant production sufficient costs are charged on regular virology testing. It is possible to avoid partly of these costs by means of keeping virus-free plants in vitro. We have investigated the influence of strawberry long-term storage on propagation and rooting capacity, appearance of non-true type regenerants, pollen fertility, yielding capacity and vegetative productivity. It was shown the relatively high stability of the main pomological characters of strawberry plants after 15 years of storage. For economical evaluations we have compared methods of deposition in vitro and the maintenance of usual field collections. For this purpose the expenses of both treatments were counted. Calculations were made for 50 varieties (20 copies per each variety, total quantity 1000 copies). According to our calculations, the storage of strawberry in vitro in refrigerating chambers is 3.2 times more economical than maintenance of field collection. Besides, there is significant economy of the soil areas. Also it is necessary to note, that stored in vitro material does not reduce the phytosanitary status. According to our researches, plants after in vitro storage produced more runner plants in comparison with traditionally propagated ones. Thus the potential... [to full text]

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Available abstract

Economical efficiency of different in vitro techniques depends on botanical species and the scale of production. We have shown that the profitability of micropropagation for commercial laboratories can achieve 160%. In system of healthy plant production sufficient costs are charged on regular virology testing. It is possible to avoid partly of these costs by means of keeping virus-free plants in vitro. We have investigated the influence of strawberry long-term storage on propagation and rooting capacity, appearance of non-true type regenerants, pollen fertility, yielding capacity and vegetative productivity. It was shown the relatively high stability of the main pomological characters of strawberry plants after 15 years of storage. For economical evaluations we have compared methods of deposition in vitro and the maintenance of usual field collections. For this purpose the expenses of both treatments were counted. Calculations were made for 50 varieties (20 copies per each variety, total quantity 1000 copies). According to our calculations, the storage of strawberry in vitro in refrigerating chambers is 3.2 times more economical than maintenance of field collection. Besides, there is significant economy of the soil areas. Also it is necessary to note, that stored in vitro material does not reduce the phytosanitary status. According to our researches, plants after in vitro storage produced more runner plants in comparison with traditionally propagated ones. Thus the potential... [to full text]

Key concepts: Environmental science, Biochemical engineering, Engineering

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