Fenitrothion 분해미생물 Bacillus sphaericus NFo1의 동정 및 분해 최적조건

A study was carried out to find out the methodology of biological treatment for wastes and wastewater caused by an organophosporus insecticide, fenitrothion, using fenitrothion-degrading microorganism. A fenitrothion-degrading microorganism was isolated by using a selective nutrient broth (NS) medium including fenitrothion, and identified to Bacillus sphaericus NFol based on its morphological and biochemical characteristics. Further, investigation was processed to determine the optimal culture conditions degrading fenitrothion in NB medium by using the NFol strain. As results, the cultural conditions determined for temperature, initial pH and inoculum for the optimum growth of the strain and degradation of fenitrothion, which has a exact co-relationship between both of them, were 35℃, 7.5 and 1.5 at OD 660 value, respectively. In this conditions, fenitrothion could be degraded within 5 days over 90% at the high concentrations of fenitrothion, upto 200 ㎎/l.