2013•European Respiratory JournalRequires access
IL-10 and IL-1ra mediates OVA-induced Th2 airway allergic response at short and long-term
Ronaldo Aparecido da Silva, Francine Maria de Almeida, Clarice Rosa Olivo, Beatriz Mangueira Saraiva-Romanholo, Adenir Perini, Mílton A. Martins, Celso Ricardo Fernandes Carvalho
Open publisher page 0 citations
Abstract
Aerobic training (AT) decreases airway allergic inflammation in sensitized mice; however the anti-inflammatory mechanisms are poorly known. Aim: Evaluate the mechanisms involved during an AT in asthma animal model.Method: 160 Balb/c mice were divided in 4 groups (n=24 each): Control (CT), AT, Ovalbumin (OVA), OVA+AT. OVA groups were sensitized with i.p. OVA+Al(OH)-3 and OVA-inhalations that continued during AT. AT begun after chronic airway inflammation, and it was performed at 50% maximal exercise capacity (60 min/session; 5xweek) and it was performed for 1, 3, 7, 15 or 30 days. After those periods, mice were euthanized and then analyzed the eosinophil counting (BALF), expression of IL-4, IL-5, NF-kB, IL-10, IL-1ra, Eotaxin, RANTES and ICAM-1 by immunohistochemistry in tissue lung slices. Results: OVA-induced an increase in eosinophil migration and the expression of Th2 cytokines, NF-kB, Eotaxin, RANTES and ICAM-1 over time (*p