Protein Production in Mammalian Cells

Abstract Originally published in: Production of Recombinant Proteins. Edited by Gerd Gellissen. Copyright © 2005 Wiley‐VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3‐527‐31036‐4 The sections in this article are Why Use Mammalian Cells for Heterologous Gene Expression? Mammalian Cell Lines for Protein Production Mammalian Expression Systems Design of the Basic Expression Unit Transient Expression and Episomal Vectors: Alternatives to Stable Integration “Stable” Integration into the Host Genome Selection Strategies for Mammalian Cells Auxotrophic Selection Markers and Gene Amplification The Integration Locus: a Major Determinant of Expression Level Mammalian Cell‐based Fermentation Processes Batch and Fed‐batch Fermentation Continuous Perfusion Fermentation Continuous Production with Hollow‐fiber Bioreactors Conclusions Acknowledgments