2014•Biomedical Spectroscopy and ImagingRequires access

Spectroscopic properties of two single-cysteine mutants of EGFP: C48S-EGFP and C70S-EGFP

Joanna Krasowska, Krastyu G. Uriginov, Patricia L. Clark, Andrzej Sienkiewicz, Agnieszka Bzowska, Beata Wielgus‐Kutrowska

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Abstract

The enhanced green fluorescent protein (EGFP) is broadly used as an intracellular fluorescent marker. Its numerous applications require a comprehensive understanding of EGFP folding properties, as well as the properties of EGFP-derived mutants. Herei

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Key concepts: Green fluorescent protein, Chemistry, Mutant, Cysteine, Cell biology, Biophysics, Biochemistry, Biology