1993•Current Protocols in ImmunologyRequires access

In Vitro Evaluation of Experimental Agents for Anti‐ HIV Activity

Douglas D. Richman, Victoria A. Johnson, Douglas M. Mayers, Takuma Shirasaka, Mary C. O'Brien, Hiroaki Mitsuya

Abstract

This unit presents an assay that has proven useful as an initial screening test is an HIV cytopathic effect (CPE) inhibition assay in which immortalized T cell lines (e.g., ATH8 or MT2) that are profoundly sensitive to the cytopathic effect of certain strains of HIV are utilized as target cells. Additional protocols assess the anti-HIV activity of certain candidate agents by measuring inhibition of syncytium formation or p24 gag protein production by ELISA. Calculation of the 50% inhibitory concentration (IC(50)) is also presented.

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Key concepts: Syncytium, Cytopathic effect, In vitro, Human immunodeficiency virus (HIV), Cell culture, Chemistry, Inhibitory postsynaptic potential, Molecular biology