Isolation and development of microsatellite markers for the Japanese dormouse, Glirulus japonicus
Shumpei P. Yasuda, Akiyoshi Nakayama, Manami Iwabuchi, Shusaku Minato, Koichiro Tsuchiya, Hitoshi Suzuki
Abstract
Shumpei P. Yasuda, Akiyoshi Nakayama, Manami Iwabuchi, Shusaku Minato, Koichiro Tsuchiya, Hitoshi Suzuki
Abstract
Eight microsatellite markers were developed for the Japanese dormouse (Glirulus japonicus), a natural monument and near-threatened species in Japan. The markers amplify in individuals from all of the mitochondrial lineages detected in a previous study. Numerous polymorphisms were detected in specimens from a local population in central Honshu (11-21 alleles per locus; n = 31) and from the entire distribution range of the species (19-41 alleles per locus; n = 152). These microsatellites will be useful in conservation genetic studies of G. japonicus.
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Eight microsatellite markers were developed for the Japanese dormouse (Glirulus japonicus), a natural monument and near-threatened species in Japan. The markers amplify in individuals from all of the mitochondrial lineages detected in a previous study. Numerous polymorphisms were detected in specimens from a local population in central Honshu (11-21 alleles per locus; n = 31) and from the entire distribution range of the species (19-41 alleles per locus; n = 152). These microsatellites will be useful in conservation genetic studies of G. japonicus.
Key concepts: Biology, Microsatellite, Locus (genetics), Threatened species, Allele, Mitochondrial DNA, Genetic marker, Population