마늘 추출물이 N-Nitrosodimethylamine의 생성에 미치는 영향

This study was conducted to investigate the effect of garlic extracts on electron donating ability, nitrite-scavenging activity and N-nitrosodimethylamine (NDMA) formation. Content of total phenolic compounds was the highest in ethyl acetate extract. Electron donating ability was significantly increased with increasing sample concentration, as 88.0±1.53% from ethyl acetate extract and 77.5±0.8% from butanol extract in concentration of 10 ㎎/mL. Butanol extract from garlic with highest nitrite scavenging activity and inhibition effect of NDMA formation exhibited 96.9±0.75% and 68.6±1.15% in concentration of 10 ㎎/mL, respectively. The butanol extract was fractionated into 5 fractions using prep-LC. Nitrite-scavenging activity and inhibition effect of NDMA formation were excellent in fraction Ⅲ, which further fractioned into 3 subfractions (Ⅲ-a, Ⅲ-b and Ⅲ-c) using prep-LC. Of the 3 subfractions, its activity was the highest in the subfraction Ⅲ-c. Therefore butanol extract of garlic was suggested to be effective inhibitor of NDMA formation.