2009Acta HorticulturaeRequires access

A PRELIMINARY STUDY ON CHLOROPLAST SIMPLE SEQUENCE REPEATS IN CASTANEA

Cao Qingqin, Kai Yang, Zhen Zhen, Yongqing Feng, Qin Ling

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Abstract

Ten previously reported chloroplast simple sequence repeats (cpSSRs) primers from Nicotiana tabacum and Arabidopsis thaliana were used to analyze the chloroplast DNA variabilities in three main Castanea species, i.e. C. mollissima, C. crenata and C. henryi. The results showed eight cpSSR primers revealed specific amplification bands, three of which produced polymorphic bands. Eight polymorphic alleles were generated using those three primers, with an average of 2.67 alleles per primer. The general universality of cpSSR primers was further discussed.

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Ten previously reported chloroplast simple sequence repeats (cpSSRs) primers from Nicotiana tabacum and Arabidopsis thaliana were used to analyze the chloroplast DNA variabilities in three main Castanea species, i.e. C. mollissima, C. crenata and C. henryi. The results showed eight cpSSR primers revealed specific amplification bands, three of which produced polymorphic bands. Eight polymorphic alleles were generated using those three primers, with an average of 2.67 alleles per primer. The general universality of cpSSR primers was further discussed.

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Available abstract

Ten previously reported chloroplast simple sequence repeats (cpSSRs) primers from Nicotiana tabacum and Arabidopsis thaliana were used to analyze the chloroplast DNA variabilities in three main Castanea species, i.e. C. mollissima, C. crenata and C. henryi. The results showed eight cpSSR primers revealed specific amplification bands, three of which produced polymorphic bands. Eight polymorphic alleles were generated using those three primers, with an average of 2.67 alleles per primer. The general universality of cpSSR primers was further discussed.

Key concepts: Simple (philosophy), Sequence (biology), Chloroplast, Computational biology, Biology, Evolutionary biology, Genetics, Philosophy

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