In Vitro Transformation Assays Using Mouse Embryo Cell Lines

Balb/c-3T3 cells have been widely applied to the routine testing of chemicals for their in vitro transforming potential. Although a number of 3T3 cell clones have been described, few are useful in the context of morphological transformation and all of those that are presently available were originally isolated by T. Kakunaga ( 7 ). The lineage of these cells has been discussed earlier, but it is important to realize that the majority of chemical screening studies have been conducted using Kakunaga’s clone 1–13 and therefore the methods described in the following sections apply specifically to this clone. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.