Molecular Cloning of Sugarcane Late Embryogenesis Abundant Protein Gene(LEA) and Its Expression Character

Late embryogenesis abundant protein gene(LEA) is an important gene in plant stress response.A full-length cDNA sequence of sugarcane Lea gene was obtained from sugarcane(Saccharum officinarum) leaf full-length cDNA library through sequencing and validated by the corresponding bioinformatics analysis, termed Sc-Lea.The full-length of Sc-Lea was 921 bp, with a 495 bp open reading frame(ORF), a 64 bp 5' untranslated region(UTR) and 362 bp 3' UTR.At the 3' end UTR, there was a clear polyA structure, the sequence around the start codon was CCCCAGCCATGGC, and the base of-3 and +4 was G, and C was the preferred base in this sequence which was in line with the rules of Kozak.Then, the prokaryotic expression vector, which contained the ORF of sugarcane Sc-Lea gene, was constructed and the targeted protein at the molecular weight 17.9 kD coding by sugarcane Sc-Lea gene was induced by IPTG.Moreover, in order to explore the expression characters of sugarcane Sc-Lea gene under the stress of SA, H2O2, PEG and NaCl, the Real-time qPCR approach was applied.The results showed that the expression of the sugarcane Sc-Lea gene was induced both by PEG and NaCl, while inhibited by H2O2.Also, the expression of this gene could be influenced by SA.It can be to some extent inferred that the sugarcane Sc-Lea gene obtained in this study plays an important role in the sugarcane drought-tolerant and salt-tolerant mechanism.