2014•Diyala Agricultural Sciences JournalOpen access

Diagnostic of wireworm that attack potato tubers by using PCR.

Reda S. Al-jorany, F. H. Sadik

Abstract

Field studies were carried out in the middle of Iraq during 2009 - 2012 to detect and identify the species of genus Agriotes spp that infest Potato tubers (Solanum tubersum L. ) by using Molecular diagnosis Polymerase Chain Reaction (PCR) for both adults and larvae . The results showed that there are five species of Agriotes infest Potato tubers in Spring and Autumn cultivation . These species are : Agriotes brevis (Candeze) , Agriotes lineatus(Linnaeus) Agriotes obscurus (Linnaeus) , Agriotes sputator(Linnaeus) Agriotes ustulatus (Schaller). A genetic diversity within the species of the genus Agriotes was found

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Key concepts: Biology, Botany, Genus, Polymerase chain reaction, Gene, Genetics