국내외 봉독의 HPLC를 이용한 멜리틴 함량 분석비교

The study was melittin content analysis of purified bee venom collected from Korean and another country. Using HPLC, A sample used the Uzbekisan, Vietnam and Korea bee venom. The experimental conditions of HPLC, the UV detection wavelength was set at 215㎚, the column temperature was 35℃, the injection volume was 20㎕, the moble phase A was 0.22% TFA solution in water and the mobile phase B was 0.2% TFA solution in acetonitrile. The content of melittin was that Uzbekistan bee venom was percentage of 20.38%, Vietnam bee venom were percentage of 36.70% and 41.41%, respectively. Therefore, Korea bee venom were percentage of 56.71%, 52.30% and 50.07%, respectively. As a result, melittin content of Korea bee venom was higher than Uzbekistan and Vietnam bee venom. These results suggest that Korea bee venom maintained high purity and another country bee venom component was change in quality by other reason. However, further studies on the specific mechanism are needed.