1989•Journal of Clinical MicrobiologyOpen access
Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever
F A Rubin, Paul D. McWhirter, N H Punjabi, Edward M. Lane, Pratiwi Sudarmono, Sri Pandam Pulungsih, Murad Lesmana, Swiandy Kumala, Dennis J. Kopecko, Stephen L. Hoffman
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Abstract
A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.