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TOBACCO MOSAIC VIRUS IN CHLOROPLAST AND CYTOPLASM OF INFECTED TOBACCO LEAF

Kamehisa Matsushita

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Abstract

Chloroplasts containing Tobacco Mosaic Virus (TMV) were isolated from TMV inoculated tobacco leaves, and TMV was extracted from them. The preparations from isolated chloroplasts of infected leaves showed 30% increase in the optical density at 260 mμ over those from healthy leaves. Most of infectivity was observed in chloroplast fraction 40 hr after inoculation. Thereafter, infectivity in the chloroplast fraction decreased and that of cytoplasm increased with time.

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What this paper is about

Chloroplasts containing Tobacco Mosaic Virus (TMV) were isolated from TMV inoculated tobacco leaves, and TMV was extracted from them. The preparations from isolated chloroplasts of infected leaves showed 30% increase in the optical density at 260 mμ over those from healthy leaves. Most of infectivity was observed in chloroplast fraction 40 hr after inoculation. Thereafter, infectivity in the chloroplast fraction decreased and that of cytoplasm increased with time.

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Available abstract

Chloroplasts containing Tobacco Mosaic Virus (TMV) were isolated from TMV inoculated tobacco leaves, and TMV was extracted from them. The preparations from isolated chloroplasts of infected leaves showed 30% increase in the optical density at 260 mμ over those from healthy leaves. Most of infectivity was observed in chloroplast fraction 40 hr after inoculation. Thereafter, infectivity in the chloroplast fraction decreased and that of cytoplasm increased with time.

Key concepts: Tobacco mosaic virus, Infectivity, Chloroplast, Tobacco leaf, Cytoplasm, Inoculation, Biology, Virology

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TOBACCO MOSAIC VIRUS IN CHLOROPLAST AND CYTOPLASM OF INFECTED TOBACCO LEAF — Research Paper | ScholarLens