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EVALUATION OF EFFECTS OF CRYOPRESERVATION ON RABBIT SPERMATOZOA MEMBRANES WITH TRYPAN BLUE-GIEMSA STAINING

Zsuzsanna Polgár, G. Virág, B. Baranyai, S. Bodó, András Kovács, Elen Gócza

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Abstract

The effects of cryopreservation on membrane of rabbit spermatozoa were studied by Trypan blue-Giemsa staining. Semen samples of 11 White New Zealand bucks (N=31) were evaluated before freezing and after freezing-thawing. The applied cryopreservation method based on two cryoprotectants (DMSO, glycerol). The integrity of the head and tail membrane and the acrosome status were evaluated on smears stained by trypan blue and Giemsa stains according to KOVACS, FOOTE (1992). The freezing-thawing process increased the number of dead cells from 18% to 46%. At the same time live cell heads were also observed and significant damage of acrosomal and tail membrane integrity (p<0.001) was detected. The most destroying effect was shown on sperm tail, which was found as the doubled number of stained tail spermatozoa. The freezingthawing effects were not influenced by collection date but some differences were detected between semen of different bucks in term of tolerance to freezing.

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The effects of cryopreservation on membrane of rabbit spermatozoa were studied by Trypan blue-Giemsa staining. Semen samples of 11 White New Zealand bucks (N=31) were evaluated before freezing and after freezing-thawing. The applied cryopreservation method based on two cryoprotectants (DMSO, glycerol). The integrity of the head and tail membrane and the acrosome status were evaluated on smears stained by trypan blue and Giemsa stains according to KOVACS, FOOTE (1992). The freezing-thawing process increased the number of dead cells from 18% to 46%. At the same time live cell heads were also observed and significant damage of acrosomal and tail membrane integrity (p<0.001) was detected. The most destroying effect was shown on sperm tail, which was found as the doubled number of stained tail spermatozoa. The freezingthawing effects were not influenced by collection date but some differences were detected between semen of different bucks in term of tolerance to freezing.

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Available abstract

The effects of cryopreservation on membrane of rabbit spermatozoa were studied by Trypan blue-Giemsa staining. Semen samples of 11 White New Zealand bucks (N=31) were evaluated before freezing and after freezing-thawing. The applied cryopreservation method based on two cryoprotectants (DMSO, glycerol). The integrity of the head and tail membrane and the acrosome status were evaluated on smears stained by trypan blue and Giemsa stains according to KOVACS, FOOTE (1992). The freezing-thawing process increased the number of dead cells from 18% to 46%. At the same time live cell heads were also observed and significant damage of acrosomal and tail membrane integrity (p<0.001) was detected. The most destroying effect was shown on sperm tail, which was found as the doubled number of stained tail spermatozoa. The freezingthawing effects were not influenced by collection date but some differences were detected between semen of different bucks in term of tolerance to freezing.

Key concepts: Trypan blue, Cryopreservation, Cryoprotectant, Giemsa stain, Staining, Acrosome, Andrology, Semen

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EVALUATION OF EFFECTS OF CRYOPRESERVATION ON RABBIT SPERMATOZOA MEMBRANES WITH TRYPAN BLUE-GIEMSA STAINING — Research Paper | ScholarLens