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1982•Journal of Clinical MicrobiologyOpen access

Enrichment medium and control system for isolation of Campylobacter fetus subsp. jejuni from stools

Francis Chan, A. M. Mackenzie

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Abstract

Enrichment culture with a semisolid medium increased by 6% the isolation rate of Campylobacter fetus subsp. jejuni. The semisolid enrichment medium was also used successfully as a transport medium for Campylobacter isolates. A blood agar plate streaked with Pseudomonas aeruginosa, Clostridium perfringens, and a laboratory strain of Campylobacter was a good control system for the microaerophilic atmosphere. Good growth of all three organisms indicated satisfactory conditions for culturing Campylobacter.

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Enrichment culture with a semisolid medium increased by 6% the isolation rate of Campylobacter fetus subsp. jejuni. The semisolid enrichment medium was also used successfully as a transport medium for Campylobacter isolates. A blood agar plate streaked with Pseudomonas aeruginosa, Clostridium perfringens, and a laboratory strain of Campylobacter was a good control system for the microaerophilic atmosphere. Good growth of all three organisms indicated satisfactory conditions for culturing Campylobacter.

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Available abstract

Enrichment culture with a semisolid medium increased by 6% the isolation rate of Campylobacter fetus subsp. jejuni. The semisolid enrichment medium was also used successfully as a transport medium for Campylobacter isolates. A blood agar plate streaked with Pseudomonas aeruginosa, Clostridium perfringens, and a laboratory strain of Campylobacter was a good control system for the microaerophilic atmosphere. Good growth of all three organisms indicated satisfactory conditions for culturing Campylobacter.

Key concepts: Campylobacter, Microbiology, Isolation (microbiology), Campylobacter fetus, Biology, Campylobacter jejuni, Agar, Microaerophile

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