Knockdown of OPN by OPN-RNAi inhibits growth and invasiveness of U87 glioma cells

Objective:To study the effect of siRNA targeting osteopontin(OPN-RNAi)on the proliferation and invasiveness of U87 glioma cells and the possible mechanism.Methods: OPN-RNAi was synthesized according to the gene sequence of OPN protein and was transfected into U87 cells.The proliferation of U87 cells was examined by MTT;matrix metalloprotease(MMP) 2 and MMP9 expression were detected by Western blotting assay;transwell assay and gelatin-zymogram were used to detect the invasion ability of U87 cells and gelatinase acitivity of MMP2 and MMP9,respectively.Results: Synthesized OPN-RNAi effectively inhibited the expression of OPN in U87 cells(P0.05).OPN-RNAi also significantly decreased the expression of MMP2 and MMP9 in U87 cells(P0.05) and the gelation activity of MMP2 and MMP9(P0.01),and inhibited the proliferation and invasivenss of U87 cells(all P0.05).Conclusion: Knockdown of OPN with OPN-RNAi can inhibit the proliferation and invasiveness of U87 cells,which is probably related to the decreased expression of MMP2 and MMP9 genes and their gelatinase acitivities.