1977•The Journal of BiochemistryRequires access

Measurement of the pH of Frozen Buffer Solutions by Using pH Indicators

Yutaka Orii, Masayuki MORITA

Abstract

A method was established to estimate the pH change of several buffers solutions on freezing by using a combination of pH indicators. Among more than 30 buffers solutions examined, almost half exhibited a pH change in the temperature range between freezing point and 220 degrees K; the results were tabulated. Glycerol was found to suppress the pH changes because of its "salt buffer" effect.

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Key concepts: Buffer (optical fiber), Chemistry, Buffer solution, Salt (chemistry), Phosphate buffered saline, Glycerol, Chromatography, Analytical Chemistry (journal)