2015•Antimicrobial Agents and ChemotherapyOpen access

Purification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays

Omaira Vera, Paula Brelas de Brito, Letusa Albrecht, Keillen Monick Martins-Campos, Paulo Filemon Paolucci Pimenta, Wuelton Marcelo Monteiro, Marcus Lacerda, Stefanie Costa Pinto Lopes, Fábio Trindade Maranhão Costa

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Abstract

Significant progress toward the control of malaria has been achieved, especially regarding Plasmodium falciparum infections. However, the unique biology of Plasmodium vivax hampers current control strategies. The early appearance of P. vivax gametocytes in the peripheral blood and the impossibility of culturing this parasite are major drawbacks. Using blood samples from 40 P. vivax-infected patients, we describe here a methodology to purify viable gametocytes and further infect anophelines. This method opens new avenues to validate transmission-blocking strategies.

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Key concepts: Gametocyte, Plasmodium vivax, Malaria, Virology, Plasmodium falciparum, Biology, Transmission (telecommunications), Plasmodium (life cycle)