Organs and Plantlets Regeneration of Gladiolus through Tissue Culture

Explants from inflorescence stalks of Gladiolus when cultured in vitro regenerated new plantlets within 6–7 weeks. Regeneration was started by the formation on the basal end of a thin layer of callus and root primordia. This was followed by formation of buds and cormlets, on the distal end. The regeneration of the various organs from the explants was found to be polarized and depended on the levels of growth substances added to the basal medium, best combination for organ initiation being 10 ppm naphthalene-acetic acid and 0.5 ppm of kinetin.