Expression of exogenous xylanase gene (atx) in transgenic rice.

For improving the production efficiency of xylanase by expression of xylanase in transgenic rice plants,a xylanase gene(atx) was inserted into the binary expression vector pCAMBIA1301,and the resulting construction with atx was named atx-Ru3ep-1301.The atx-Ru3ep-1301 vector was introduced into rice(Oryza sativa L.subsp.japonica) variety Zhonghua 11 via Agrobacterium-mediated transformation,by using the rice mature embryo-derived callus as the explants.Both hygromycin resistance detection and PCR amplification confirmed that the target gene had been integrated into the genome of transgenic rice.RT-PCR analysis of the total RNA extracted from the fresh leaves of several transgenic lines showed that the introduced xylanase gene could be normally expressed in rice under the control of CaMV 35S promoter.And in the leaves of transgenic rice,xylanase activity was up to 4.37 U/g in fresh leaf.In addition,growth and reproduction of the transgenic rice plants were not affected by the expression of foreign xylanase.Thus,it would be an economic way for xylanase production via transgenic rice.