1988Journal of BacteriologyOpen access

Sufficiency of the Klenow fragment for survival of polC(Ts) pcbA1 Escherichia coli at 43 degrees C

S K Bryan, R E Moses

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Abstract

Escherichia coli cells with the pcbA1 allele and temperature-sensitive DNA polymerase III mutations survived at a restrictive temperature if DNA polymerase I activity was present. The Klenow fragment of DNA polymerase I was capable of supporting cell survival as well.

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Escherichia coli cells with the pcbA1 allele and temperature-sensitive DNA polymerase III mutations survived at a restrictive temperature if DNA polymerase I activity was present. The Klenow fragment of DNA polymerase I was capable of supporting cell survival as well.

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Available abstract

Escherichia coli cells with the pcbA1 allele and temperature-sensitive DNA polymerase III mutations survived at a restrictive temperature if DNA polymerase I activity was present. The Klenow fragment of DNA polymerase I was capable of supporting cell survival as well.

Key concepts: Klenow fragment, DNA polymerase I, Biology, Escherichia coli, DNA polymerase, Molecular biology, Polymerase, DNA polymerase II

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