Sufficiency of the Klenow fragment for survival of polC(Ts) pcbA1 Escherichia coli at 43 degrees C
S K Bryan, R E Moses
Abstract
S K Bryan, R E Moses
Abstract
Escherichia coli cells with the pcbA1 allele and temperature-sensitive DNA polymerase III mutations survived at a restrictive temperature if DNA polymerase I activity was present. The Klenow fragment of DNA polymerase I was capable of supporting cell survival as well.
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Escherichia coli cells with the pcbA1 allele and temperature-sensitive DNA polymerase III mutations survived at a restrictive temperature if DNA polymerase I activity was present. The Klenow fragment of DNA polymerase I was capable of supporting cell survival as well.
Key concepts: Klenow fragment, DNA polymerase I, Biology, Escherichia coli, DNA polymerase, Molecular biology, Polymerase, DNA polymerase II