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2010•Journal of the South China Agricultural UniversityRequires access

A full-length cDNA subtractive hybridization (FLSSH) method and its application in the isolation of inducible expression genes in rice.

Qunyu Zhang, Yao‐Guang Liu

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Abstract

cDNA suppression subtractive hybridization(SSH) has been used to analyze differential gene expression widely,but there are some shortcomings in it such as difficult mRNA preparation,difficult mRNA handling and short cDNA fragments to be obtained.A Full-length cDNA SSH(FLSSH) method based on SSH and SMART cDNA synthesis method was used to obtain differentially-expressed genes of rice treated by probenazole(PBZ).By northern blot analysis,two up-regulated and one down-regulated candidate cDNA fragments by PBZ were identified.

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cDNA suppression subtractive hybridization(SSH) has been used to analyze differential gene expression widely,but there are some shortcomings in it such as difficult mRNA preparation,difficult mRNA handling and short cDNA fragments to be obtained.A Full-length cDNA SSH(FLSSH) method based on SSH and SMART cDNA synthesis method was used to obtain differentially-expressed genes of rice treated by probenazole(PBZ).By northern blot analysis,two up-regulated and one down-regulated candidate cDNA fragments by PBZ were identified.

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Available abstract

cDNA suppression subtractive hybridization(SSH) has been used to analyze differential gene expression widely,but there are some shortcomings in it such as difficult mRNA preparation,difficult mRNA handling and short cDNA fragments to be obtained.A Full-length cDNA SSH(FLSSH) method based on SSH and SMART cDNA synthesis method was used to obtain differentially-expressed genes of rice treated by probenazole(PBZ).By northern blot analysis,two up-regulated and one down-regulated candidate cDNA fragments by PBZ were identified.

Key concepts: Suppression subtractive hybridization, Complementary DNA, Northern blot, Biology, Molecular biology, Gene, Gene expression, cDNA library

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A full-length cDNA subtractive hybridization (FLSSH) method and its application in the isolation of inducible expression genes in rice. — Research Paper | ScholarLens