2007•Current Protocols in Nucleic Acid ChemistryRequires access

Biophysical Analysis of Triple‐Helix Formation

Alexandre S. Boutorine, Christophe Escudé

Abstract

Two methods for DNA triple-helix analysis are described in this unit: a gel-shift assay based on the slower electrophoretic migration of a triplex in a polyacrylamide gel under nondenaturing conditions, and an optical method in which the thermal denaturation of the triple helix is followed by UV spectrophotometry. Both methods give valuable information on the characteristics of DNA triple-helix formation and triplex stability under different conditions.

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Key concepts: Triple helix, Helix (gastropod), Denaturation (fissile materials), Electrophoresis, DNA, Polyacrylamide gel electrophoresis, Chemistry, Thermal stability