098 High Regeneration Ability of Benzyladenine and Naphthalene Acetic Acid Induced Callus of Eastern Hibiscus

The ability of Hibiscus syriacus explants to produce regenerable callus was investigated. Fragments of cotyledons, hypocotyl, and roots were cultured on MS media supplemented with two different auxins (2,4-D and NAA, both 0.3 mg/L) and three different cytokinin (BA, 2iP and kinetin, all 0.1 mg/L). Plants were regenerated on McCown media with three different cytokinins at two different concentrations (0.1 and 1.0 mg/L). The biggest volume of callus was produced on medium containing 2,4-D/2iP (2,821 mm 3 /explant). The smallest mass of callus was induced on medium with NAA/kinetin (120 mm 3 /explant). On BA-supplemented media, the auxin type had no significant influence on the amount of callus produced. The highest number of shoots and leaves was produced on callus induced on NAA/BA supplemented media and regenerated on medium with 0.1 mg/L BA (4.2 shoots and 20 leaves/explant). Callus cultured on medium with 1.0 mg/L of BA produced significantly less shoots (2.4 shoots/explant). The lowest number of shoots was observed on callus originating from NAA/kinetin and NAA/2iP callus media and grown on medium with kinetin or 2ip (both 1.0 mg/L). The highest number of roots was produced by cultures originating from NAA/BA callus medium grown on the BA regeneration medium, irrespective of the cytokinin level. The longest shoots were observed on medium supplemented with 1.0 mg/L BA (18 mm). In this study, the best plant regeneration results were obtained for callus initiated on NAA/BA supplemented medium and regenerated on medium with 0.1 mg/L BA, however, the highest production of callus was observed on medium with 2,4-D/2iP.