Existence of two levels of repression in the biosynthesis of methionine in Saccharomyces cerevisiae: effect of lomofungin on enzyme synthesis

Derepression of a methionine biosynthetic enzyme (homocysteine synthase) has been studied after repression either by exogenous methionine or by exogenous S-adenosylmethionine (SAM). Lomofungin, which inhibits the synthesis of ribosomal precursor and messenger ribonucleic acid but not of protein in Saccharomyces cerevisiae, has been used in this system. It has been shown that the addition of this antibiotic prevents the derepression of homocysteine synthase after repression by exogenous methionine but not after repression by exogenous SAM. These experiments with lomofungin and the kinetics of repression after addition of methionine or SAM to the growth medium provide evidence that the repression induced by exogenous methionine acts at the transcriptional level whereas the repression induced by exogenous SAM acts at the translational level.