2009•PubMedRequires access
[Studies on the apoptosis of RK13 cells induced by rabbit hemorrhagic disease virus].
Ni Zheng, Wei Wei, Guangqing Liu, Liu Chen, Bin Yu, Tao Yun, Jionggang Hua, LI Shuang-mao
Open publisher page 6 citations
Abstract
The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining, DNA ladder, Caspase 3 activity and flow cytometry, etc. The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei. As shown in the paper, a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h, 48h and 72h post-infection, confirming that DNA fragmentation was induced by RHDV infection. The results of flow cytometry showed that about 63% of cells were in apoptosis at 48h post-infection. Besides, we also demonstrated that the activation of Caspase 3 occurred during the infection process. In conclusion, our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.