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Isolation of Alternaria alternata from Wheat Crop Spike Let

Prabhat K. Mishra, Firoz Naem Khan

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Abstract

The best growth of Alternaria alternata was observed on Malt Yeast agar medium with a colony diameter of 2cm after 4 days of incubation. The decline phase started from the 12th day of incubation and favored acidic as well as neutral pH range. At alkaline ph, hydrogen ion concentration had inhibitory effect on the growth and development of mycelia and pigmenta- tion of the fungus. Best growth had been observed at a wide range of temperature between 20-35oC and sporulation was observed at 28oC, and 95 to 100% RH proved optimum.

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What this paper is about

The best growth of Alternaria alternata was observed on Malt Yeast agar medium with a colony diameter of 2cm after 4 days of incubation. The decline phase started from the 12th day of incubation and favored acidic as well as neutral pH range. At alkaline ph, hydrogen ion concentration had inhibitory effect on the growth and development of mycelia and pigmenta- tion of the fungus. Best growth had been observed at a wide range of temperature between 20-35oC and sporulation was observed at 28oC, and 95 to 100% RH proved optimum.

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Available abstract

The best growth of Alternaria alternata was observed on Malt Yeast agar medium with a colony diameter of 2cm after 4 days of incubation. The decline phase started from the 12th day of incubation and favored acidic as well as neutral pH range. At alkaline ph, hydrogen ion concentration had inhibitory effect on the growth and development of mycelia and pigmenta- tion of the fungus. Best growth had been observed at a wide range of temperature between 20-35oC and sporulation was observed at 28oC, and 95 to 100% RH proved optimum.

Key concepts: Alternaria alternata, Incubation, Mycelium, Spore, Agar, Biology, Yeast, Yeast extract

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