Mature embryo as a source material for efficient regeneration response in sorghum (Sorghum bicolor L. Moench.).

Efficient plant regeneration is a prerequisite for a complete genetic transformation protocol in cereals.Aiming this, in the present study, we have accomplished efficient plant regeneration using mature embryos as a source material in Sorghum bicolor (L.) Moench.Although immature inflorescence and immature embryos are best explant sources for in vitro culture in Sorghum, however they are available only for a limited period in a year.Mature embryos have always been ideal for in vitro studies for the reason that they can be handled easily over other explants and available throughout the year.Mature embryo explants of Sorghum bicolor genotypes viz.IS 3566, SPV 475, CSV13, CSV15, CSV112, IS 348 were cultured on MS medium for efficient callus induction and subsequent plant regeneration.The response of different combination and concentrations of plant growth regulators were compared, and factors affecting the mature embryo tissue culture response were studied in this manuscript.Significant genotypic differentiation was detected in embryogenic callus induction and plantlet regeneration.Genotype IS 3566 showed better tissue culture response than the other genotypes.Efficient embryogenic callus induction was achieved with 2mg l -1 2, 4,5-Trichlorophenoxyacetic acid (2,4,5-T) and multiple shoot induction was achieved by manipulation of 6-benzyl adenine (BAP), Thidiazuron (TDZ), and Indole-3-acetic acid (IAA) in the culture medium.