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Detection of deletion mutations at the gpt locus in pSV2gpt transformed CHO cells
Kenneth R. Tindall, Abraham W. Hsie
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Abstract
Deletion mutations were detected at the gpt locus in HGPRT-deficient CHO cells transformed by the pSV2gpt plasmid vector. The purine analog, 6-thioguanine (TG), is metabolized by XGPRT and was shown to be applicable for the isolation of mutants at the gpt locus in one pSV2gpt transformed CHO cell line carrying a single copy of the gpt gene. Induction of TG/sup r/ mutants in this cell line by x-rays was quantifiable. Specific deletions of pSV2gpt sequences in two TG/sup r/ mutants were detected by blot hybridization. The sites of these depletions provide a molecular basis for the observed TG/sup r/ phenotype. 17 references.