Control of Haptoglobin Metabolism. III

1) The Hb-Hp “subunit” and the Hb-Hp complex were purified from human blood of the Hp 1-1 type by ammonium sulfate fractionation, gel filtration on a Sephadex G-100 column, and chromatography on a DEAE-sephadex column. Each purified sample was homogeneous in the ultracentrifuge. Disc electrophoresis, however, revealed the presence of a minor contaminant less than 3% of the total protein.2) From the data obtained on disc electrophoresis, it was suggested that a reversible change from the Hb-Hp complex to the Hb-Hp “subunit” does not occur at least in vitro.3) On the basis of the several evidences obtained, it was concluded that the Hb-Hp “subunit II” is identical with the Hb-Hp complex.