Minimum inhibitory concentration of kanamycin of four varieties of Colombian rice calli

Kanamycin (Km) is an antibiotic that has been used in the production of transgenic rice plants as a selective agent. In this study, we evaluated the antibiotic sensitivity of seed derived callus in four varieties of colombian rice (FEDEARROZ 2000, FEDEARROZ 50, 369 and CICA FEDEARROZ 8). It was induced callus formation from mature sedd in a specific medium, it were resulting in percentages of callus formation greater than 90%. Later callus were subsequently taken to a regeneration medium, which are important variations in the rate of regeneration, between 62% (F2000) and 11% (F369). Seven different concentrations of kanamycin (0 mg/L, 100 mg/L, 200 mg/L, 300 mg/L, 400 mg/L, 500 mg/L and 600 mg/L) were tested added to the regeneration medium. It was observed differential response to the antibiotic. F369 to F2000 and from 500 mg/L showed no regenerating, while for F50 and CICA 8 was from 300 mg/L.