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Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Melaleuca alternifolia (tea tree oil).

Christine Carson, Katherine A. Hammer, Thomas V. Riley

Open publisher page 191 citations

Abstract

A broth micro-dilution method was used to examine the susceptibility of Escherichia coli (n = 110) and Staphylococcus aureus (n = 105) to the essential oil of Melaleuca alternifolia (tea tree oil). The detergent Tween 80 was used successfully to enhance the solubility of tea tree oil in the test medium. The MIC90 of tea tree oil for E. coli was 0.25% while for S. aureus it was 0.50%.

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What this paper is about

A broth micro-dilution method was used to examine the susceptibility of Escherichia coli (n = 110) and Staphylococcus aureus (n = 105) to the essential oil of Melaleuca alternifolia (tea tree oil). The detergent Tween 80 was used successfully to enhance the solubility of tea tree oil in the test medium. The MIC90 of tea tree oil for E. coli was 0.25% while for S. aureus it was 0.50%.

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OpenAlex reports 191 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

A broth micro-dilution method was used to examine the susceptibility of Escherichia coli (n = 110) and Staphylococcus aureus (n = 105) to the essential oil of Melaleuca alternifolia (tea tree oil). The detergent Tween 80 was used successfully to enhance the solubility of tea tree oil in the test medium. The MIC90 of tea tree oil for E. coli was 0.25% while for S. aureus it was 0.50%.

Key concepts: Melaleuca alternifolia, Tea tree oil, Staphylococcus aureus, Escherichia coli, Essential oil, Melaleuca, Microbiology, Biology

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Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Melaleuca alternifolia (tea tree oil). — Research Paper | ScholarLens