Crown gall of grapevine in Slovenia

42 samples of grapevine plants and propagating material were taken in 2 Slovene vine growing districts and tested for the presence of Agrobacterium sp. 31 samples had suspicious symptoms, the other 11 did not have them. The samples were analysed using 4 methods: growth of agrobacteria on 4 semiselective media, detection of T-DNA carrying oncogenes tmr, acs and vis/6b by PCR, detection of opines in tumor tissue and pathogenicity testing on tomato and Kalanchoe daigremontiana plants. 20 samples with symptoms were proved to be infected with Agrobacterium vitis by means of molecular method and 19 by detection of opines. A. vitis was also proved in 3 samples without symptoms. 19 isolates induced tumor formation on tested plants. This is the first evidence of A.vitis in Slovenia.