2014•The Journal of Animal and Plant SciencesRequires access
Micropropagation of Jartorpha curcas L. through shoot tip explants using different concentrations of phytohormones.
Muhammad Imtiaz, Abdul Mateen Khattak, Nargis Ara, Ayesha Iqbal, Hafeez Ur Rahman
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Abstract
A study was conducted to evaluate the effect of phytohormone BAP alone and in combination with IBA or NAA for invitro
propagation of Jatropha curcas shoot tip explants. MS medium supplemented with various concentrations of
phytohormones significantly affected shoot induction. Maximum (93.33%) shoot induction occurred when MS medium
was supplemented with 8.88 μM BAP or 13.32 and 4.92 μM of BAP and IBA respectively. Similarly, maximum shoot
buds per explant (6.7) and shoot length (4.5 cm) were obtained when 13.32 μM BAP and 4.92μM IBA was added to the
MS medium. Shoots produced were re-cultured to MS, ½ MS media and ½ MS medium supplemented with different
concentrations of IBA and were studied for rooting, where the response was highly significant. IBA supplemented to ½
MS medium at 14.76μM gave maximum (4.93) roots per culture