The Role of Phytoalexins in Plant Protection

Plants resist pathogens with combinations of constitutive and induced defences. Of the induced defences, phytoalexin production has received much attention since the phytoalexin concept was introduced over 50 years ago. However, the specific role of phytoalexins in disease resistance is not clear for the majority of host-parasite systems. Much of the research on phytoalexins has relied on the identification of induced antifungal compounds and correlating their presence with resistance. Although an important first step, more definitive studies are needed. Approaches that use in situ localization and quantification have provided good evidence that phytoalexins can accumulate at the right time, concentration, and location to be effective in resistance. Studies on phytoalexin tolerance in pathogenic fungi have also shown a relationship between virulence and the ability of fungi to detoxify phytoalexins. Use of mutants deficient in phytoalexin synthesis and elucidating biosynthetic pathways provide other approaches to evaluating the role of phytoalexins. These approaches will be illustrated using the Arabidopsis-camalexin system and other selected examples. Studies on natural variation in phytoalexin production and the accumulation of phytoalexins under field conditions will be presented as another means of evaluating the role of these compounds in resistance to pathogens and other pests.