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2006•Unpublished venueRequires access

Modulation of Gene Expression by 3'‐UTR Regions in Yeast: a Promoter‐Independent Alternative

S. Seoane‐Rosende, Angelina Rodríguez, María Ángeles Freire-Picos

Open publisher page 1 citations

Abstract

Changing the 3' untranslated region (3'-UTR) of the KICYC1 gene allows modulation of gene expression at the transcriptional level in two yeast species, Kluyveromyces lactis and Saccharomyces cerevisiae. These relative mRNA changes are achieved without altering the promoter or any growth condition. The comparative analysis performed revealed higher mRNA expression with the KICYC1 distal 3'-UTR region than with the proximal region in S. cerevisiae. As an application to modulate gene expression by changing the 3'-UTR region, an expression plasmid was constructed to clone coding regions under GAL1 promoter and using KICYC1 distal 3'-UTR as terminator sequence.

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What this paper is about

Changing the 3' untranslated region (3'-UTR) of the KICYC1 gene allows modulation of gene expression at the transcriptional level in two yeast species, Kluyveromyces lactis and Saccharomyces cerevisiae. These relative mRNA changes are achieved without altering the promoter or any growth condition. The comparative analysis performed revealed higher mRNA expression with the KICYC1 distal 3'-UTR region than with the proximal region in S. cerevisiae. As an application to modulate gene expression by changing the 3'-UTR region, an expression plasmid was constructed to clone coding regions under GAL1 promoter and using KICYC1 distal 3'-UTR as terminator sequence.

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Available abstract

Changing the 3' untranslated region (3'-UTR) of the KICYC1 gene allows modulation of gene expression at the transcriptional level in two yeast species, Kluyveromyces lactis and Saccharomyces cerevisiae. These relative mRNA changes are achieved without altering the promoter or any growth condition. The comparative analysis performed revealed higher mRNA expression with the KICYC1 distal 3'-UTR region than with the proximal region in S. cerevisiae. As an application to modulate gene expression by changing the 3'-UTR region, an expression plasmid was constructed to clone coding regions under GAL1 promoter and using KICYC1 distal 3'-UTR as terminator sequence.

Key concepts: Untranslated region, Coding region, Terminator (solar), Kluyveromyces lactis, Three prime untranslated region, Gene, Biology, Gene expression

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