2015International Journal of Advanced Veterinary Science and TechnologyOpen access

Effect of Different Freezing Rates and Thawing Methods on the Quality of Frozen Boar Semen

Nipu Deka, K. Ahmed, Dhruba Jyoti Kalita, Deka B.C.

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Abstract

A total of 30 ejaculates from three Hampshire crossbred boars were used to study the effect of freezing rates and thawing methods on quality of frozen boar semen. Semen was frozen using three freezing rates-30 0 c/min, 50 0 c/min and 70 0 c/min in a programmable biological semen freezer. The semen characteristics viz., sperm motility, live sperm, HOST reacted spermatozoa and intact acrosome were studied after thawing using three different thawing methods viz., 37 0 c for 30 sec, 50 0 c for 12 sec and 70 0 c for 10 sec. Out of three different freezing rates and thawing methods freezing rate 30 0 c/min with thawing method 70 0 c for 10 sec resulted better semen quality.

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What this paper is about

A total of 30 ejaculates from three Hampshire crossbred boars were used to study the effect of freezing rates and thawing methods on quality of frozen boar semen. Semen was frozen using three freezing rates-30 0 c/min, 50 0 c/min and 70 0 c/min in a programmable biological semen freezer. The semen characteristics viz., sperm motility, live sperm, HOST reacted spermatozoa and intact acrosome were studied after thawing using three different thawing methods viz., 37 0 c for 30 sec, 50 0 c for 12 sec and 70 0 c for 10 sec. Out of three different freezing rates and thawing methods freezing rate 30 0 c/min with thawing method 70 0 c for 10 sec resulted better semen quality.

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Available abstract

A total of 30 ejaculates from three Hampshire crossbred boars were used to study the effect of freezing rates and thawing methods on quality of frozen boar semen. Semen was frozen using three freezing rates-30 0 c/min, 50 0 c/min and 70 0 c/min in a programmable biological semen freezer. The semen characteristics viz., sperm motility, live sperm, HOST reacted spermatozoa and intact acrosome were studied after thawing using three different thawing methods viz., 37 0 c for 30 sec, 50 0 c for 12 sec and 70 0 c for 10 sec. Out of three different freezing rates and thawing methods freezing rate 30 0 c/min with thawing method 70 0 c for 10 sec resulted better semen quality.

Key concepts: Semen, Acrosome, BOAR, Sperm, Andrology, Bull semen, Sperm motility, Semen quality

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Effect of Different Freezing Rates and Thawing Methods on the Quality of Frozen Boar Semen — Research Paper | ScholarLens