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Pancreatic Lipase and Colipase. An Example of Heterogeneous Biocatalysis

M. Séaméariva, P. Desnuelle

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Abstract

Introduction Molecular Properties of Lipase Influence of the Physical State of the Substrate on Lipase Activity and Interfacial Activation Emulsified Substrates Action of Lipase on Substrate Monolayers The Mechanism of Lipase Action as Studied with Soluble Substrates and Inert Interfaces The Lipase–Colipase System Interactions among Lipase, Colipase, and Bile Salts in Aqueous Solution Chemical Labeling of Lipase. Structure-Functio Relationships Concluding Remarks

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Introduction Molecular Properties of Lipase Influence of the Physical State of the Substrate on Lipase Activity and Interfacial Activation Emulsified Substrates Action of Lipase on Substrate Monolayers The Mechanism of Lipase Action as Studied with Soluble Substrates and Inert Interfaces The Lipase–Colipase System Interactions among Lipase, Colipase, and Bile Salts in Aqueous Solution Chemical Labeling of Lipase. Structure-Functio Relationships Concluding Remarks

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Available abstract

Introduction Molecular Properties of Lipase Influence of the Physical State of the Substrate on Lipase Activity and Interfacial Activation Emulsified Substrates Action of Lipase on Substrate Monolayers The Mechanism of Lipase Action as Studied with Soluble Substrates and Inert Interfaces The Lipase–Colipase System Interactions among Lipase, Colipase, and Bile Salts in Aqueous Solution Chemical Labeling of Lipase. Structure-Functio Relationships Concluding Remarks

Key concepts: Colipase, Lipase, Chemistry, Substrate (aquarium), Pancreatic lipase, Triacylglycerol lipase, Biocatalysis, Enzyme

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