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Advanced Techniques in Fluorescence Spectroscopy

Bernard Valeur

Abstract

Time-resolved fluorescence in the femtosecond time range: fluorescence up-conversion technique Advanced fluorescence microscopy Improvements in conventional fluorescence microscopy Confocal fluorescence microscopy Two-photon excitation fluorescence microscopy Near-field scanning optical microscopy (NSOM) Fluorescence lifetime imaging spectroscopy (FLIM) Time-domain FLIM Frequency-domain FLIM Confocal FLIM (CFLIM) Two-photon FLIM Fluorescence correlation spectroscopy Conceptual basis and instrumentation Determination of translational diffusion coefficients Chemical kinetic studies Determination of rotational diffusion coefficients Single-molecule fluorescence spectroscopy General remarks Single-molecule detection in flowing solutions Single-molecule detection using advanced fluorescence microscopy techniques

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Available abstract

Key concepts: Fluorescence correlation spectroscopy, Fluorescence cross-correlation spectroscopy, Fluorescence-lifetime imaging microscopy, Microscopy, Fluorescence spectroscopy, Fluorescence in the life sciences, Fluorescence, Spectroscopy