Isolation of Deoxyribonuclease II of Rat Liver Lysosomes

Abstract Although deoxyribonuclease II (acid DNase) is a widely distributed lysosomal enzyme, the liver enzyme has not been highly purified or well characterized. A procedure is presented for purifying the rat liver lysosomal enzyme 43,000-fold, employing chromatographic fractionations of an extract made from lysosomes. The purified enzyme is free of all other lysosomal enzymes assayed, including phosphatases, other nucleases, and, especially, phosphodiesterase, which has frequently been reported in DNase preparations.