Metabolic inhibitors and nucleotide turnover. I. Inhibition of uptake of radiophosphate in rat liver and hepatoma slices.

Summary The purine analog, 2,6-diaminopurine, and the pyrimidine nucleoside analog, 5-aminouridine, have been shown to reduce the incorporation of radioactive phosphate into the phospholipids and the ribonucleic acid nucleotides of slices of rat liver and a rat hepatoma. 5-Aminouridine appeared somewhat more effective in liver than in hepatoma, while 2,6-diaminopurine was more effective in hepatoma. Inhibition by aminouridine was nearly uniform in all fractions in both tissues, while that by diaminopurine was greater in the purine nucleotides in liver and in adenylic acid in hepatoma. Differences between the metabolism of liver and hepatoma slices have been observed. Furthermore, purine and pyrimidine analogs have each been shown to inhibit incorporation into both purine and pyrimidine nucleotides, as well as that into phospholipids.