The effect of thyroid hormone on heme oxygenase-1 expression after liver ischemia repefusion injury in rat

Objective To explore the effect of thyroid hormone on heme oxygenase-1(HO-1)expression after liver 　　ischemia reperfusion injury in rat.Methods An ischemia-reperfusion(I-R)process of 1 h ischemia and then 6 h reperfusion 　　was established in male Sprague-Dawley rat.The 30 rats were randomly divided into three groups;sham- 　　operated,control,treatment.Treatment of 01 mg/kg intraperitoneal injection of T3 at 48 h before ischemic.The levels of 　　ALT,AST in serum and malondialdehyde(MDA)and superoxide dismutase(SOD)in liver tissue were detected at reperfusion for 6 　　hours.The expression of HO-1 were examined by Western blot and RT-PCR.Results Compared to the control group，the 　　expression of the HO-1mRNA and protein was higher in the treatment group.The level of ALT,AST in serum samples and MDA in 　　liver of the treatment group were significantly lower than that of the control group(P<005)and the level of SOD was higher 　　(P<005).Histology showed an severe necrosis in the control group.The necrosis was ameliorated in the treatment 　　group.Conclusions These results indicate that thyroid hormone up-regulate the expression of HO-1 and decrease the liver 　　ischemic reperfusion injury.