In-Vitro propagation of dahlia through shoot tip culture

Sterilized explants (shoot tip) of two dahlia cultivars under aseptic condition were inoculated on MS medium supplemented with various concentrations of growth regulators viz., BAP (1.0 and 2.0 mg/l), IAA (0.1 and 0.2 mg/l) and Kinetin (2.0, 4.0, and 8.0 mg/l). Minimum days taken for shoot initiation, higher number of shoot with better shoot length were recorded on MS medium containing 0.1 mg/l IAA and 4.0 mg/l Kinetin in both the cultivars. However, maximum dry weight of shoot was recorded with higher concentration of BAP 2.0 mg/l and lower concentration of IAA 0.1mg/l and also with lower concentration of Kinetin 2 mg/l and IAA 0.1 mg/l.